Analysis of the FLT3-ITD ratio as a prognostic factor in acute myeloid leukemias

First cases studied in Uruguay

  • Evangelina González Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Departamento Básico de Medicina, Laboratorio de Citometría y Biología Molecular. Licenciada en Bioquímica
  • Sofía Grille Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Departamento Básico de Medicina. Prof. Adjunta
  • Valeria Vales Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Cátedra de Hematología, Posgrado de Hematología
  • Matilde Boada Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Departamento Básico de Medicina. Asistente
  • Lorena M. Zanella LEB laboratorio. Bahía Blanca, Argentina. Lic. en Bioquímica
  • Daniel Leal Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Cátedra de Hematología. Asistente
  • Nehuen P. Gasparini LEB laboratorio. Bahía Blanca, Argentina. Lic. en Bioquímica
  • Cecilia Guillermo Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Cátedra de Hematología. Prof. Agregada
  • Evangelina E. Agriello Universidad Nacional del Sur, Cátedra de Hematología Clínica, Prof. Agregada. LEB laboratorio, Directora
  • Mariana Stevenazzi Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Cátedra de Hematología. Prof. Agregada
  • Daniela Lens Universidad de la República, Facultad de Medicina, Hospital de Clínicas, Departamento Básico de Medicina, Laboratorio de Citometría y Biología Molecular. Prof. Agregado
Keywords: ACUTE MYELOID LEUKEMIA, FLT3-ITD, CYTOGENETIC ANALYSIS

Abstract

Introduction: In recent years, significant progress has been made in the biological knowledge of acute myeloid leukemia (AML), which has been reflected on treatment of affected patients being guided by cytogenetics and molecular profiling. FLT3 internal tandem duplications (FLT3/ITDs) represent the most frequent mutations in AML and confer a bad prognosis in patients with intermediate cytogenetic risk. It has been reported that the presence of a high FLT3-ITD ratio (relationship between number of ITD carrier allele and wild type allele).

Objective: To standardize a technique, still not available in Uruguay, to determine the FLT3-ITD ratio in patients carriers of AML of intermediate cytogenetic risk. To discuss the first cases of AML FLT3+ who underwent ratio analysis.

Methods: In order to identify FLT3-ITD, the fragment corresponding to exons 14 and 15 of the gene was amplified in bone marrow samples upon debut of the disease. In the cases it was positive, the FLT3-ITD ratio was determined by the analysis of fragments with capillary electrophoresis.

Results: This study presented the standardization of a method to determine the FLT3-ITD ratio and the first cases analysed in our country. Twelve patients were studied and the presence of FLT3-ITD was detected in three of them. In two cases, the FLT3-ITD ratio found was below 0.8 and in one case it was greater than or equal to 0.8.

Conclusions: We have a technique to determine the FLT3-ITD ratio with an important prognostic value for patients carriers of AML.

References

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Published
2016-09-30
How to Cite
1.
González E, Grille S, Vales V, Boada M, Zanella LM, Leal D, Gasparini NP, Guillermo C, Agriello EE, Stevenazzi M, Lens D. Analysis of the FLT3-ITD ratio as a prognostic factor in acute myeloid leukemias. Rev. Méd. Urug. [Internet]. 2016Sep.30 [cited 2024Nov.8];32(3):145-51. Available from: http://www2.rmu.org.uy/ojsrmu311/index.php/rmu/article/view/162

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