Assessment of fine needle puncture-aspiration assessment (PAAF) for the diagnosis of Tuberculous lymphadenitis in patients with human immunedeficiency virus (HIV)
Abstract
Introduction: fine needle puncture aspiration (PAAF) of the lymph node is a simple, fast and low cost technique to obtain samples for diagnosis.
Objective: the objective of the study was to validate PAAF for the diagnosis of tuberculous lymphadenitis in patients with HIV, by using culture for Mycobacodeterium tuberculosis in node biopsy or histopathology as "gold standard", together to the response to the specific treatment.
Method: prospective clinical-pathological study. Patients included in our study: were infected with HIV, provided their informed consent, were older than 18 years of age, had superficial adenomagaly larger than 2 cm, and were seen at the Infectious Diseases Service from October 2004 through December 2006. Fine needle puncture aspiration (PAAF ) and biopsy of the same node were performed. Usual stain and Ziehl-Neelsen stain were used in cytological and histopatological samples, and microbacteria cultures were made.
We defined the following citology suggested TB lymphadenitis: 1) reactive lymphadenitis with necrosis; 2) reactive lymphadenitis with epithelial cells and/or macrophagos and/or multinuclear giant cells; with or without acid-alcohol resistant bacilli in the direct (sample?) with Ziehl-Neelsen stain.
Results: out of 30 patients included in the study, 23 corresponded to node TB. In the remaining seven patients, diagnosis was negative in six of them, according to PAAF, and thus unnecessary treatment was avoided, and histological study was determined for final diagnosis. Sensitivity, specificity, positive and negative predictive value of PAAF for the diagnosis of TB lymphadenitis was 95%, 86%, 95% and 86% respectively. Kappa concordance coefficient between both procedures was 90%.
Conclusions: lymph node PAAF in patients with HIV is a high performance diagnostic technique for the diagnosis of node TB. Likewise, it enables selecting patients to undergo node biopsy for final diagnose, avoiding its systematic conduction in all patients with adenomegaly.
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